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Mouse fibrosarcoma L929 R2 (TNF + cycloheximide induced) library (LMBP LIB12)
| Library name: | Mouse fibrosarcoma L929 R2 (TNF + cycloheximide induced) library |
| Library type: | cDNA |
| Library accession number: | LMBP LIB12 |
| Organism: | Mus musculus |
| Priming method: | Random (bidirectional) |
| Vector name: | pGAD424 |
| Vector sequence file: | p3106.txt |
| Vector circular map: | p3106.pdf |
| Vector accession number: | Clontech |
| Vector type: | yeast two hybrid vector |
| Cloning sites: | EcoRI |
| Library coverage: | 2,5 x 10E6 |
| Average insert size: | 1,00 kb |
| Distributed as: | pooled library DNA |
| DNA concentration: | 150 ng/µl |
| Volume: | 5 µl |
| Further information: | The host strain Escherichia coli K12 MC1061 or DH5α can be used for electroporation to amplify the library. The transformation mixture can then be streaked on selective solid medium (LB-Lennox + 100 µg/ml ampicillin) and cultivated at 37°C to obtain the different library clones. |
| History of deposit: | The cDNA library was deposited by Prof. R. Contreras1. 1 Department of Molecular Biology, Ghent University, Belgium |
| Library reference: | De Valck et al., FEBS Lett. 384 (1996), 61-64 [PMID: 8797804] |
| Related reference: | Vanhaesebroeck et al., Cancer Res. 51 (1991), 2469-2477 [PMID: 1849793] Van de Craen et al., FEBS Lett. 403 (1997), 61-69 [PMID: 9038361] Heyninck et al., J. Cell Biol. 145 (1999), 1471-1482 [PMID: 10385526] |
| Restricted distribution: | - BCCM MTA |
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